Volume 24

Long Non-coding RNA SNHG17 Exacerbates Spinal Cord Injury via the miR-381-3p/RAP1B Axis Ming Jin 1, Wentao Wu 1, Zheyong Jia 1, Hu Qin 1, Yongxin Wang 1* Corresponding author: Yongxin Wang Email: xjdwyx2000@sohu.com Mailing Address: Department of Neurosurgery, Xinjiang Medical University Affiliated First Hospital Xinyiroad 373 street， Urumqi City, Xinjiang China. AbstractObjective: Severe traumas that affect The spinal cord and the central nervous system are known as spine injuries. LongNon-Coding RNAs (lncRNAs), which act as epigenetic adjusters, have the ability to affect neuronal apoptosis after SCI, the local inflammation environment, neuropathic pain, and vascular regeneration. Therefore, the research’s first purpose is to investigate the expression mode of lncRNA SNHG17 under the condition of SCI. Thus, it also intends to confirm SNHG17’s regulatory action in apoptosis and inflammation during SCI’s progression. Hence, The mechanism-based effects of SNHG17 will be verified through further in vivo and in vitro tests. Methods: We first constructed a spinal cord injury cellular model by means of lipopolysaccharide-activated BV-2 microglial cells. We applied this method to measure the expression level of SNHG17. Additionally, A mouse brain injury model was developed to evaluate motor function using the Basso, Beattie, Bresnahan locomotor rating scale, footprint analysis, and grid-walking tests. Several functional tests were implemented in BV2 cells where SNHG17 or miR-381-3p had been modified, incorporating flow cytometry, west blot analysis, the use of the use of quantitative and the Cell Signalling Kit-8 assay. For spinal cord injury mice, adeno-associated virus was used to knock down SNHG17/RAP1B, Subsequently, histopathological evaluations were performed using haematoxylin and eosin staining, Terminal nucleotide transfers dUTP nick end labeling staining with Nissl exposure. Western blots and qualitative DNA sequencing were used for molecular studies, and behavioral tests were performed. Hence, these assessments were conducted for the purpose of clarifying the in-vivo role of SNHG17. Results: In spinal cord injury mice and BV2 cells treated with lipopolysaccharide, SNHG17 showed a very obvious upregulation. Therefore, under this situation, SNHG17 had the function to promote inflammation and apoptosis that are triggered by lipopolysaccharide. From the angle of mechanism, SNHG17 served as a competing endogenous RNA, which can sequester miR-381-3p. Thus, this action removed the inhibitory effect on RAP1B, which is the target gene of miR-381-3p. The SNHG17/miR-381-3p/RAP1B axis caused the worsening of microglial inflammation and neuronal impairment. Hence, when people carry out genetic removal of SNHG17, it can lead to the alleviation of motor deficits, the reduction of spinal cord inflammation, and the mitigation of tissue damage in spinal cord injury mice. Conclusion: The long non-coding RNA (lncRNA) SNHG17 exacerbates the effects of neurological damage, which influences the miR-381-3p/RAP1B axis. Targeted inhibition of SNHG17 represents a potential therapeutic strategy to ameliorate SCI-associated motor dysfunction and neuroinflammation. Keywords: Long non-coding RNA SNHG17; miR-381-3p/RAP1B axis; Spinal cord injury; Neuroinflammation; Apoptosis Introduction A brain injury is a dangerous illness that impairs the autonomic, voluntary, and nervous systems. Acute SCI is typically resulting from sudden outside force acting on the spine, which leads to vertebrae fracture and displacement [1, 2]. Up to 25 recorded secondary injury mechanisms have been identified following spinal cord injury (SCI), according to previous research.. Therefore, in recent period, researchers have developed various treatment methods that aim at the primary cellular and molecular pathophysiological changes in SCI. These changes include secondary injury mechanisms, the damage of broken spinal cord neuronal circuits, and the important functional losses caused by SCI [4, 5].a range of medications or techniques with neuroprotective characteristics are being developed to tackle secondary damage processes, such as factors related to inflammation and apoptosis [6, 7]. Furthermore, other research has concentrated on modifying the signaling molecules that regulate neuronal development in order to promote the regeneration of severed spinal cord tracts [8, 9]. Non-coding ribonucleic acid (ncRNA) refers to RNA molecules which do not undertake protein-coding tasks. It mainly includes long non-coding ribonucleic acid (lncRNA), micro-ribonucleic acid (miRNA), as well as other unclassified RNA types. Recent study work has pointed out that spinal cord injury (SCI) can cause changes in lncRNA gene expression levels, and lncRNAs have an extremely important functional status in SCI. However, the mechanism through which lncRNA adjusts the expression of SCI-related genes remains not clear [10, 11]. A wide number of experimental investigations have convincingly revealed that there are considerable variations in lncRNA gene expression in situations involving spinal cord injury(SCI). The exact method by which long noncoding RNAs (lncRNAs) function in spinal cord damage is yet unknown. Therefore, gaining a more deep-level understanding about the function of lncRNA in SCI will be helpful for the establishment of SCI treatment methods. A large number of research investigations have confirmed that microRNAs (miRNAs) occupy a very important status in the secondary injury processes of spinal cord injury (SCI). These processes include inflammation, angiogenesis, axonal regrowth, and glial cell development [13 – 17]. miRNAs control the expression of related proteins. They realize this goal by either raising or reducing the expression of target genes that have changes after SCI, therefore participating in the pathophysiological events of SCI . It must be stressed that the regulatory function of miRNA after SCI is complex and diversified. It involves multiple targets and signaling pathways, it is also impacted by several circumstances. Hence, future researches should make deeper exploration into the regulatory mechanisms of miRNA in SCI. This exploration will provide new concepts and methods for the treatment of SCI. At present, the research about the influence of lncRNA upon spinal cord injury is in a limited state. The aim of this work aims to examine SNHG17 levels of expression in spinal cord injury (SCI) and its regulatory function in flare and apoptosis during SCI. Additionally, the study will use in vitro and in vivo tests to confirm SNHG17’s legal operations. New molecular targets for clinical SCI treatment will be made available by further elucidation of the molecular mechanisms of SNHG74 in the development of spinal cord damage. 2 Materials and Methods 2.1 Experimental Animal Model The female C57BL/6 mice utilized in this research weighed between 25 and 30 grams. The mice were supplied by the Animal Experiment … Read more

Impact of establishing an integrated working group of medical and nursing staff in the endoscopy unit on the course, complications, and prognosis of gastrointestinal endoscopic procedures First Author (Chinese): Yao Jianhong, Female, Head Nurse, Bachelor’s Degree, Department of Gastroenterology, Shanxi Bethune Hospital, No. 99 Longcheng Street, Xiaodian District, Taiyuan City, Shanxi Province, yjh19821001@163.com/030032 Pinyin: Jianhong Yao Co-first Author (Optional): Miao Huali, Female, Associate Chief Nurse, Master’s Degree, Nursing Department, Shanxi Bethune Hospital, No. 99 Longcheng Street, Xiaodian District, Taiyuan City, Shanxi Province, 574420441@qq.com Pinyin: Huali Miao 2nd Author: Wang Na, Female, Assistant Researcher, PhD Candidate, Department of Gastroenterology, Shanxi Bethune Hospital, No. 99 Longcheng Street, Xiaodian District, Taiyuan City, Shanxi Province. Nawangsxu@163.com/030032 Pinyin: Na Wang 3rd Author: Jing Xiying, Female, Associate Chief Nurse, Bachelor’s Degree, Department of Gastroenterology, Shanxi Bethune Hospital, No. 99 Longcheng Street, Xiaodian District, Taiyuan City, Shanxi Province. 847893656@qq.com/030032 Pinyin: Xiying Jing 4th Author: Zheng Rong, Female, Head Nurse, Bachelor’s Degree, Department of Gastroenterology, Shanxi Bethune Hospital, No. 99 Longcheng Street, Xiaodian District, Taiyuan City, Shanxi Province. 2804750570@qq.com/030032 Pinyin: Rong Zheng 5th Author: Ning Yijun, Male, Head Nurse, Department of Gastroenterology, Shanxi Bethune Hospital, No. 99 Longcheng Street, Xiaodian District, Taiyuan City, Shanxi Province, nyj15034169@163.com/030032 Pinyin: Yijun Ning 6th Author: Zhu Qingjiao, Male, Nurse, Bachelor’s Degree, Department of Gastroenterology, Shanxi Bethune Hospital, No. 99 Longcheng Street, Xiaodian District, Taiyuan City, Shanxi Province, 525799921@qq.com/030032 Corresponding Author: Chai Bao, Male, Chief Physician, Department of Gastroenterology, Shanxi Bethune Hospital Department of Gastroenterology, Shanxi Bethune Hospital, No. 99 Longcheng Street, Xiaodian District, Taiyuan City, Shanxi Province, chaibaocb@126.com/030032 Pinyin: Bao Chai If supported by a grant, please specify the grant name and grant number (this will not be added after acceptance): 202403021212191 Research on the mechanism by which neck ring structure affects the colonization of enterohemorrhagic Escherichia coli through regulation of T3SS protein components. Abstracts Objective To evaluate the impact of establishing an integrated work group of endoscopy unit providers on the course of gastrointestinal endoscopic procedures, complications, and prognosis. Methods Patients who underwent gastrointestinal endoscopic surgical treatment in our hospital from January 2023 to December 2023 were recruited, and whether or not they received integrated workgroup management by endoscopy unit medical staff was used as an exposure factor to form two cohorts of routine management and workgroup management, and to compare surgical process, complications, and prognosis of different cohorts. Results A total of 128 patients who underwent gastrointestinal endoscopic surgical treatment were included, of which 61 cases were in the work group management cohort and 67 cases in the routine management cohort. Compared with the conventional management cohort, the working group management cohort had shorter operation time and gastrointestinal function recovery time (P<0.05); compared with the conventional management cohort, the working group management cohort had a lower complication rate (P<0.05); compared with the group before treatment, the scores of the Quality of Life Evaluation Scale (SF-36) of the two groups after treatment were significantly higher, and the working group management cohort’s SF-36 scores were better than those of the conventional management cohort (P<0.05). Conclusion Establishment of an integrated work group of endoscopy unit providers may speed up the process of gastrointestinal endoscopy, reduce complications and improve prognosis. Keywords Integrated working group of healthcare professionals; Gastrointestinal endoscopy; Surgical process; Complications; Introduction Minimally invasive gastrointestinal endoscopy has become an important treatment method in gastroenterology due to its simple operation, less invasive, faster recovery and fewer complications, etc. It is widely used in the treatment of gastrointestinal bleeding, stenosis, tumors, and other diseases through direct observation by gastrointestinal endoscopy to accurately determine the nature and extent of lesions1 . Gastrointestinal endoscopy is a high-risk procedure performed by professionals, and as an invasive procedure, patients are prone to stress, leading to reduced compliance with treatment2 . Conventional nursing interventions are useful in helping patients understand gastrointestinal endoscopy, including providing guidance on preparation for the examination, explaining the procedure, and informing patients of possible complications, but they are ineffective in improving patient comfort during endoscopic treatment and are difficult to alleviate through simple explanations and guidance3,4 . Healthcare integration model is an innovative medical working model, which breaks down the traditional healthcare state of clear boundaries between doctors and nurses, and closely integrates doctors, nurses and patients to form a whole working together5 . Integrated medical care model refers to the process in which doctors and nurses with certain professional knowledge and competence provide healthcare services to patients under the premise of mutual trust, democratic equality, through objective joint decision-making, open communication and coordination, and shared responsibility6 . Therefore, our hospital used the establishment of endoscopy room medical and nursing staff integration work group to intervene in patients with gastrointestinal endoscopy diagnosis and treatment, to observe the intervention situation and to conduct a study, which is now reported as follows. Data Source of cases Patients who underwent gastrointestinal endoscopic procedures at our institution from January 2023 to December 2023 were recruited to form two cohorts, routine management and work group management, using as an exposure factor whether or not they received integrated work group management by endoscopy unit medical staff. Inclusion criteria (1) endoscopic mucosal resection, endoscopic submucosal dissection, endoscopic varicose vein ligation, endoscopic injection sclerotherapy, and endoscopic retrograde cholangiopancreatography at our institution; (2) cognitively normal; (3) first-time endoscopy patients; and (4) signed informed consent. Exclusion criteria (1) those with infectious diseases; (2) those with blood disorders; (3) those with organ insufficiency; and (4) those taking sedative drugs for a long time. Removal criteria (1) patients who did not complete the follow-up visit, including those who withdrew on their own and those who missed the visit; and (2) those who could not complete all the observation items for any reason. Study Subgroups and Intervention Programs Study Subgroups Two cohorts of included patients undergoing gastrointestinal endoscopic surgical treatment were formed based on whether or not the patients were managed by an integrated workgroup of endoscopy unit providers during the perioperative period: a routine management cohort versus a workgroup management cohort. Intervention programs Routine management cohort: (1) preoperative: inform the patient of the diagnosis and treatment precautions, help the patient to complete the preoperative preparation; (2) intraoperative: pay … Read more